UNAIR NEWS – Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by a virus known asdengue. Its prevalence, according to WHO, was recorded at 75% in the Asia Pacific region from burden ofdengue in the world, between 2004 and 2010.
Meanwhile, Indonesia was reported as the second country with the largest DHF cases among 30 other endemic region countries. In 2015, the number of dengue fever patients in 34 provinces in Indonesia was 129,179, and 1,240 of which died.
One DHF drug that has been found is an organic compound called MAC (Melaleuca alternifolia Concetrate), which is an active antiviral dengue obtained from endemic plant extracts in Autralia, Melaleuca alternifolia.
However, the drug has several disadvantages, which are expensive and inefficient production process. These weaknesses can be overcome by preventing the spread and looking for effective drugs for dengue fever.
In their research, Universitas Airlangga students who are members of an Exact Research Student Creativity Program (PKM-PE) team conducted a study entitled “The Effectiveness of Morin Compound from Extract of Psidium guajava Against Complex Co(II) Compound as a Drug Candidate for Dengue Hemorrhagic Fever. “
Under the direction and guidance of UNAIR lecturers, Harsasi Setyawati, S.Si., M.Sc., the proposal prepared by three UNAIR students Anisa Maharani, Yovilianda Maulitiva Untoro, and Novia Faridatus Sholihah successfully passed the selection and was entitled to a research funding from the Ministry of Research, Technology and Higher Education in the 2018 PKM program.
The purpose of this follow-up study is, among others, to develop methods of synthesizing complex Co (II)-morin compound. It also aims to determine the characteristics of complex Co (II)-morin compound using UV-Vis spectrophotometer, FTIR, and Ficher-John Melting Point Apparatus. In addition, it aims to test the effectiveness of complex Co (II)-morin compound as an antiviral drug for dengue by testing activity and toxicity.
The research was conducted for four months in the Inorganic Chemistry Laboratory, Faculty of Science and Technology (FST) UNAIR, including at the FST Analytical Chemistry Laboratory, as well as at Institute of Tropical Desease (ITD) of Universitas Airlangga.
In a previous study conducted by Soeprapto Ma’at, guava leaf extract (Psidium guajava) was proven to be able to increase the number of platelets with dengue fever, making it appropriate for the treatment of dengue fever.
But Guava can only increase the amount of Hb in the body, so a dengue virus inhibitor which comes from complex compounds needs to be developed. The synthesis of complex compounds is relatively easier when compared to the synthesis of inorganic materials and organic compounds.
The morphine content of guava and cobalt compounds has a correlation between each other so that a complex compound, namely Co (II)-morin was made as a candidate for DHF medicine.
Characterization that was carried out to test the effectiveness of complex Co (II) -morin compound covers UV-Vis spectrophotometer, FTIR, and Ficher-John Melting Point Apparatus, activity test and toxicity test showed that the Co (II)-morin compound has a potential to become a candidate for dengue fever medicine.
The conclusion in this Unair PKM-PE study is that it is Co (II) -morin complex with the characterization of activity tests and toxicity having IC 50 of 9,467 µg / mL and CC 50 of 3.36 μg / ml and indicating that the Co (II) -morin complex has a potential to be a candidate for antiviral agent DENV-1 inhibitor because it is toxic to vero cells. (*)
Editor: Bambang Bes
Source: Unair News